seqgen Command
Sequelizer’s seqgen subcommand creates physical signals from textual DNA sequences.
It can read sequences from FASTA/FASTQ files or generate random sequences (i.e., ACGT strings), and simulate the nanopore signals that would be produced when those sequences are processed by a nanopore sensor. You might have seen a basic example in Examples.
Selecting reads
Use the --select option to pick out the reads that you want to generate on (you only need quotes if you use special characters like spaces and *).
- Name pattern:
--select "6ea6609b"or--select 6ea6609b - Multiple names:
--select "6ea6609b,7b02d4c4"or--select 6ea6609b,7b02d4c4 - Single index:
--select "1"or--select 1 - Full range:
--select "101:110"or--select 101:110 - Open-ended:
--select "10:"or--select 10: - Start range:
--select ":12"or--select :12 - Multiple selections:
--select "1,3,5"or--select 1,3,5 - Mixed:
--select "1:2,8:12"or--select 1:2,8:12